2016

BiotINK

Two years ago the iGEM Team Munich at the lab of Prof. Dr. Arne Skerra managed to win the grand price in the Overgraduate Segment of the iGEM Competition.

  • Grand Prize Winner
  • Best Manufacturing Project prize
  • Best Hardware prize
  • Best Software Tool prize
  • Best New Composite Part nomination
  • Best Model nomination
  • Best Entrepreneurship nomination
  • Best Presentation nomination
Muc16_Team_Stage

Abstract

The supply of donor organs for medical transplantation is decreasing. To confront this pressing issue, we developed a game-changing approach to bioprint tissues for biomedical applications. Our interdisciplinary work aims to create a unique ink, named bio(t)INK, to revolutionize bioprinting. The printing process uses a hijacked 3D printer and two components of biotINK to induce an instantaneous polymerization reaction, creating three-dimensional multi-cellular structures in a user-definable manner. The principle of this two-component glue relies on the rapid and specific interaction of biotin and its tetrameric binding protein avidin. To make use of this high biotin-avidin affinity for cell-cell cross-linking, we engineered cells presenting biotin moieties or biotin-binding proteins on their surfaces as well as recombinant matrix proteins, which co-polymerize upon printing. Furthermore, we explored genetic circuits which allow us to functionalize the bio-synthetic tissue and install biosafety mechanisms. Altogether, we are confident that our system provides the necessary means to advance the SynBio community to the next level – the tissue level.

visit their page for more information: Team TUM/LMU Munich 2016

© iGEM Munich 2018